![]() ![]() Although discovered only relatively recently, this last category of RNA function has been shown to play a prominent role in posttranscriptional and transcriptional gene regulation. RNA molecules perform some of the most fundamental and important functions in organisms, including coding for proteins (mRNAs), providing structural support (tRNAs, lncRNAs), acting as vital enzymes (snRNAs, ribozymes), and guiding proteins to regulate other RNAs (miRNAs, piRNAs, siRNAs). CLASH Analyst is freely available for academic use. We demonstrate that CLASH Analyst can identify miRNA- and piRNA-targeting sites reported from published CLASH data and should be applicable to analyze other RNA–RNA interactions. Detailed annotation of the captured RNA–RNA interactions is then presented for the user to visualize within the server or download for further analysis. CLASH Analyst accepts raw CLASH data as input and identifies the RNA chimeras containing the regulatory and target RNAs according to the user’s interest. Here, we present CLASH Analyst, a web server that can analyze raw CLASH data within a fully customizable and easy-to-use interface. In addition, many published CLASH studies do not show the full scope of RNA–RNA interactions that were captured, highlighting the importance of reanalyzing published data. However, analyzing CLASH data requires scientists to use advanced bioinformatics, and currently available tools are limited for users with little bioinformatic experience. The recent development of the crosslinking, ligation, and sequencing of hybrids (CLASH) method has allowed scientists to map transcriptome-wide RNA–RNA interactions by identifying chimeric reads consisting of fragments from regulatory RNAs and their targets. Non-coding RNAs, such as miRNAs and piRNAs, play critical roles in gene regulation through base-pairing interactions with their target molecules.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |